MtDNA deletions, respiratory chain enzyme complexes and ATP synthesis were divided determined with polymerase chain reaction ( PCR), enzyme kinetics and bioluminescence technique. 心肌线粒体DNA缺失、线粒体呼吸链酶复合体及腺苷三磷酸(ATP)合成分别用聚合酶链反应(PCR)、酶动力学和生物发光技术进行测定。
Improvement of allicin oil extractive technique by enzyme treatment and its reaction kinetics 酶法提高大蒜油得率及其反应动力学研究
The kinetics of lipase-catalyzed esterification of oleic acid and dodecanol in hexane was investigated. The effect of enzyme and substrate concentration on the initial rate of esterification was studied, and the reaction kinetics was described in terms of the Michaelis-Menton mechanism. 在正己烷中应用工业用脂肪酶催化油酸和正十二醇的酯合成反应,研究了该反应的动力学机制,考察了酶和底物浓度对反应初速度的影响。
The results of this paper show that a three state enzyme catalysis model can fit the saccharifying of corn starch by diastase. In addition, this reaction has characteristics of single substrate reaction kinetics. 发现玉米淀粉的酶糖化反应,可以用酶的三态模型描述,而且该反应具有单底物酶动力学特征。